Author: Yeung, Siu-Wai; Lee, Thomas Ming-Hung; Cai, Hong; Hsing, I-Ming
Title: A DNA biochip for on-the-spot multiplexed pathogen identification Document date: 2006_9_25
ID: 0sg0hv9w_23
Snippet: We have demonstrated the utilization of a siliconÀglassbased microchamber for DNA-based detection of E.coli and B.subtilis cells. Thermal cell lysis, magnetic particle-based target genome isolation, DNA amplification, and electrochemical sequence-specific amplicons detection have been successfully implemented in this microdevice platform. The portable electrochemical instrumentation as well as a simple microchip design is conducive to the realiz.....
Document: We have demonstrated the utilization of a siliconÀglassbased microchamber for DNA-based detection of E.coli and B.subtilis cells. Thermal cell lysis, magnetic particle-based target genome isolation, DNA amplification, and electrochemical sequence-specific amplicons detection have been successfully implemented in this microdevice platform. The portable electrochemical instrumentation as well as a simple microchip design is conducive to the realization of on-site pathogen detection. The selective immobilization of capture probes using the pyrrole-based electropolymerization process provides good thermal stability and PCR process compatibility, which are crucial for the multiplexed analysis. Future work will be directed towards interfacing the microchip with macro-world so as to achieve a fully automatic device that can be used by untrained individuals.
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