Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain Document date: 1996_7_2
ID: 45x96b5d_40
Snippet: To determine which mechanism of ER localization, retention or retrieval, utilizes these two domains in Sec12p, we carried out metabolic pulse-labeling and chase experiments on the chimeric proteins. Since Ochlp, yeast etl-->6 mannosyl transferase, is located in the early Golgi compartment, the rate of al-->6 mannose modification should reflect the rate of transport from the ER to the early Golgi......
Document: To determine which mechanism of ER localization, retention or retrieval, utilizes these two domains in Sec12p, we carried out metabolic pulse-labeling and chase experiments on the chimeric proteins. Since Ochlp, yeast etl-->6 mannosyl transferase, is located in the early Golgi compartment, the rate of al-->6 mannose modification should reflect the rate of transport from the ER to the early Golgi.
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