Selected article for: "cytoplasmic domain and vacuolar staining"
Title: Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain
  • Document date: 1996_7_2
    • ID: 45x96b5d_50
    Snippet: Since the cytoplasmic domain of Sed4p is homologous to that of Secl2p, we tested its effect on the ER localization as well. 4DDm (Fig. 12 A) was expressed in wild-type cells, and indirect immunofluorescence microscopy was performed (Fig. 12 B) . 4DDm was mainly located in the ER, but weak vacuolar staining was also observed in some cells. This is very similar to SDDm and SDD, suggesting that the cytoplasmic domain of Sed4p also has an ability to .....
    Document: Since the cytoplasmic domain of Sed4p is homologous to that of Secl2p, we tested its effect on the ER localization as well. 4DDm (Fig. 12 A) was expressed in wild-type cells, and indirect immunofluorescence microscopy was performed (Fig. 12 B) . 4DDm was mainly located in the ER, but weak vacuolar staining was also observed in some cells. This is very similar to SDDm and SDD, suggesting that the cytoplasmic domain of Sed4p also has an ability to localize the molecule in the ER in a similar manner to that of Secl2p.

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