Author: Poe, Jonathan C.; Kountikov, Evgueni I.; Lykken, Jacquelyn M.; Natarajan, Abirami; Marchuk, Douglas A.; Tedder, Thomas F.
Title: EndoU is a novel regulator of AICD during peripheral B cell selection Document date: 2014_1_13
ID: 5804sjmo_39
Snippet: Purified spleen B cells (B Cell isolation kit; Miltenyi Biotec) were analyzed by flow cytometry for blast development after 48 h of culture with F(ab) 2 goat anti-mouse IgM Abs as previously described (Poe et al., 2004) . Alternatively, B cell proliferation during 72-h cultures was assessed by 3 H-thymidine incorporation or CFSE labeling and dilution as previously described (Poe et al., 2004) ......
Document: Purified spleen B cells (B Cell isolation kit; Miltenyi Biotec) were analyzed by flow cytometry for blast development after 48 h of culture with F(ab) 2 goat anti-mouse IgM Abs as previously described (Poe et al., 2004) . Alternatively, B cell proliferation during 72-h cultures was assessed by 3 H-thymidine incorporation or CFSE labeling and dilution as previously described (Poe et al., 2004) .
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