Selected article for: "cDNA clone and genetic marker"
Author: Almazán, Fernando; DeDiego, Marta L.; Sola, Isabel; Zuñiga, Sonia; Nieto-Torres, Jose L.; Marquez-Jurado, Silvia; Andrés, German; Enjuanes, Luis
Title: Engineering a Replication-Competent, Propagation-Defective Middle East Respiratory Syndrome Coronavirus as a Vaccine Candidate
  • Document date: 2013_9_10
    • ID: 14yfs4pa_19
    Snippet: The full-length sequence of rMERS-CoV, recovered from the infectious cDNA clone, was completely identical to that published for the original MERS-CoV-EMC12 isolate (15) , except for the silent point mutation introduced as a genetic marker. Therefore, both viruses should have the same biological properties. In fact, the growth kinetics and CPE caused by both viruses were similar when the same multiplicity of infection (MOI) was used to infect Huh-.....
    Document: The full-length sequence of rMERS-CoV, recovered from the infectious cDNA clone, was completely identical to that published for the original MERS-CoV-EMC12 isolate (15) , except for the silent point mutation introduced as a genetic marker. Therefore, both viruses should have the same biological properties. In fact, the growth kinetics and CPE caused by both viruses were similar when the same multiplicity of infection (MOI) was used to infect Huh-7 cells (14) .

    Search related documents:
    Co phrase search for related documents
    • cDNA clone and infectious cDNA clone: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cDNA clone and length sequence: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • cDNA clone and point mutation: 1, 2, 3, 4, 5, 6, 7, 8
    • cDNA clone and virus cause: 1
    • genetic marker and infectious cDNA clone: 1, 2, 3, 4, 5
    • genetic marker and length sequence: 1, 2
    • genetic marker and point mutation: 1, 2, 3
    • infection multiplicity and MOI infection multiplicity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • infection multiplicity and point mutation: 1
    • infection multiplicity and virus cause: 1, 2, 3
    • infectious cDNA clone and length sequence: 1, 2, 3, 4
    • infectious cDNA clone and point mutation: 1, 2, 3, 4, 5, 6, 7
    • length sequence and point mutation: 1, 2
    • length sequence and virus cause: 1, 2
    • MOI infection multiplicity and virus cause: 1
    • point mutation and virus cause: 1