Title: Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular transport step Document date: 1994_1_1
ID: 3xixqqsz_55
Snippet: These data indicate that the M protein is first synthesized as an unglycosylated precursor in the ER and which subsequently acquires the intermediate compartment modification, GalNAc, under conditions where vesicular transport would be expected to be blocked. The complete absence of Figure 15 . Between 14.2 and 15°C the M protein acquires GalNAc. The M protein was pulse-labeled for 2 rain at 37°C and chased for 60 and 120 min at the indicated t.....
Document: These data indicate that the M protein is first synthesized as an unglycosylated precursor in the ER and which subsequently acquires the intermediate compartment modification, GalNAc, under conditions where vesicular transport would be expected to be blocked. The complete absence of Figure 15 . Between 14.2 and 15°C the M protein acquires GalNAc. The M protein was pulse-labeled for 2 rain at 37°C and chased for 60 and 120 min at the indicated temperatures in DME/10% FCS, 2 mM methionine and 10 mM Hepes-KOH. As a reference for the M~ form, cells were also chased under ATP-depleting conditions at 37°C as before.
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