Title: Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular transport step Document date: 1994_1_1
ID: 3xixqqsz_6
Snippet: In the study by Tooze et al. (1988) using sac(-) cells it was Shown that, at 31°C, MHV was able to bud, but the assembled virus stayed in the budding compartment and was not transported through the Golgi to the extracellular medium in the normal manner. Under these conditions the M protein acquired only GalNAc, suggesting that GalNAc is added in the budding compartment. Therefore, GalNAc is a potential marker for this structure. Similarly, the p.....
Document: In the study by Tooze et al. (1988) using sac(-) cells it was Shown that, at 31°C, MHV was able to bud, but the assembled virus stayed in the budding compartment and was not transported through the Golgi to the extracellular medium in the normal manner. Under these conditions the M protein acquired only GalNAc, suggesting that GalNAc is added in the budding compartment. Therefore, GalNAc is a potential marker for this structure. Similarly, the presence of Gal and SA to the newly synthesized M protein provides evidence for its transport to the Golgi complex.
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