Author: Wilfred, Eneku; Mutebi, Francis; Mwiine, Frank Norbert; James, Okwee-Acai; Lonzy, Ojok
Title: Porcine Circovirus type 2 – Systemic disease on pig farms and associated knowledge of key players in the pig industry in Central Uganda Document date: 2018_8_28
ID: 6wkxhrwl_19
Snippet: Amplification of target DNA was done using QIAGEN HotStar HiFidelity PCR Kit as per the manufacturer's protocol. Briefly, 5 µL of sample DNA template was added to 10 PCR conditions included initial activation step of heating at 94°C for 5 mins, denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C and the cycle from denaturation to extension was repeated for 40 cycles, then final extension at 72°C for 5 min and end of.....
Document: Amplification of target DNA was done using QIAGEN HotStar HiFidelity PCR Kit as per the manufacturer's protocol. Briefly, 5 µL of sample DNA template was added to 10 PCR conditions included initial activation step of heating at 94°C for 5 mins, denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extension at 72°C and the cycle from denaturation to extension was repeated for 40 cycles, then final extension at 72°C for 5 min and end of PCR cycling at 4°C. 5 µL of the amplified products were analyzed by electrophoresis (120v for 1 h) on 1.5% (w/v) agarose gels and visualized under UV light after staining with ethidium bromide and photographs taken.
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