Selected article for: "recombinant protein and SDS PAGE analysis"
Author: MINAMI, Shohei; TERADA, Yutaka; SHIMODA, Hiroshi; TAKIZAWA, Masaki; ONUMA, Mamoru; OTA, Akihiko; OTA, Yuichi; AKABANE, Yoshihito; TAMUKAI, Kenichi; WATANABE, Keiichiro; NAGANUMA, Yumiko; KANAGAWA, Eiichi; NAKAMURA, Kaneichi; OHASHI, Masanari; TAKAMI, Yoshinori; MIWA, Yasutsugu; TANOUE, Tomoaki; OHWAKI, Masao; OHTA, Jouji; UNE, Yumi; MAEDA, Ken
Title: Establishment of serological test to detect antibody against ferret coronavirus
  • Document date: 2016_3_3
    • ID: 3g75spkc_16
    Snippet: The plasma of No.10 and serum of ferret No.22 showed differ-ent reactivities from the other samples in ELISA (Fig. 2) . To confirm the different antigenicity, immunoblot analysis was carried out using serum of ferret No.22. Plasma of ferret No.48 was used to compare with serum of ferret No.22. The purified proteins were confirmed to be single bands by cBB staining after SDS-PAGE analysis and used (Fig. 3A) . Plasma of ferret No.48 and serum of fe.....
    Document: The plasma of No.10 and serum of ferret No.22 showed differ-ent reactivities from the other samples in ELISA (Fig. 2) . To confirm the different antigenicity, immunoblot analysis was carried out using serum of ferret No.22. Plasma of ferret No.48 was used to compare with serum of ferret No.22. The purified proteins were confirmed to be single bands by cBB staining after SDS-PAGE analysis and used (Fig. 3A) . Plasma of ferret No.48 and serum of ferret No.22 reacted with recombinant protein GST-N (1-179), but only plasma of ferret No.48 also reacted with GST-N (180-374) ( Fig. 3B and 3c) . The results of the immunoblot analysis were consistent with those of the ELISA.

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