Author: Jang, Guehwan; Kim, Seong-Hee; Lee, Yoo Jin; Kim, Seungjoon; Lee, Du Sik; Lee, Kyoung-Ki; Lee, Changhee
Title: Isolation and characterization of Korean porcine deltacoronavirus strain KNU16-07 Document date: 2018_7_26
ID: 5i8q2rdf_8
Snippet: The entire genomes of the cell culture-adapted KNU16-07-P5 and -P10 strains were 25,422 nucleotides long and displayed genomic organization identical to all previously sequenced PDCoVs. The full-length genomic sequences of the original fecal sample (KNU16-07-feces, GenBank accession No. KY364365) and cell-passaged viruses were comparatively analyzed; the data are summarized in Table 1 . Compared to KNU16-07-feces, KNU16-07-P5 and -P10 identically.....
Document: The entire genomes of the cell culture-adapted KNU16-07-P5 and -P10 strains were 25,422 nucleotides long and displayed genomic organization identical to all previously sequenced PDCoVs. The full-length genomic sequences of the original fecal sample (KNU16-07-feces, GenBank accession No. KY364365) and cell-passaged viruses were comparatively analyzed; the data are summarized in Table 1 . Compared to KNU16-07-feces, KNU16-07-P5 and -P10 identically showed five nucleotide differences at positions 7737, 8218, 16481, 19808, and 21875. All mutations were non-synonymous, causing two, one, and two amino acid changes in the ORF1a, ORF1ab, and S coding regions, respectively. The two amino acid substitutions at positions 162 (Thr to Asn) and 851 (Asn to Ser) in the S coding region were sustained through passage 30 (KNU16-07-P30). No cell culture-adapting mutations arose in the E, M, and N protein-coding regions. These results reveal that the PDCoV isolate KNU16-07 is genetically stable during cell passages. This is the first report describing the isolation, serial passages, in vitro and in vivo phenotypic features, and genotypic traits of a Korean PDCoV strain. The availability of this Korean isolate will enable cutting-edge research to investigate the molecular virology as well as to develop potential vaccines against emergent PDCoVs. The information presented in this study provides fundamental knowledge that should stimulate further basic and applied studies to improve our understanding of PDCoV.
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