Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus

Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.

Several canonical pathways were highlighted by Ingenuity Pathways Analysis as being disrupted in HRSV-infected cells, including those involved in mitochondrial dysfunction (39 of a possible 172 molecules; p value, 5.89 ϫ 10 Ϫ29 ), ubiquinone biosynthesis (15 of 119 molecules; p value, 6.69 ϫ 10 Ϫ29 ), and RAN signaling (six of a possible 23 molecules; p value, 1.87 ϫ 10 Ϫ6 ). Other identified cellular proteins altered in HRSV-infected cells included components of subnuclear structures such as the nucleolus (nucleolin and nucleophosmin) and ND10s (e.g. TAR DNA protein), the latter of which had been previously highlighted as changing in HRSV-infected cells (33) , and cell cycle regulatory molecules. A number of proteins that were ablated could be grouped into specific disease associations, including respiratory disease (22 proteins ; p values, 6.84 ϫ 10 Ϫ4 -2.59 ϫ 10 Ϫ2 ), the inflammatory response (12 proteins; p values, 5.59 ϫ 10 Ϫ4 -4.56 ϫ 10 Ϫ2 ), and infectious disease (73 proteins; p values, 5.81 ϫ 10 Ϫ10 -2.59 ϫ 10 Ϫ2 ). For example, six proteins were associated with pneumonitis, which can be a feature of HRSV infection (42) .