Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_24
Snippet: Sample preparation was carried out as described above. MNP-mAb conjugates were added to 500 ïL of the processed samples for 5 min at room temperature while shaking. After the reaction solution was magnetically removed from the supernatant using a magnet, which was discarded, ENR was dissociated from the MNP-mAb conjugatesby adding 100% methanol (500 ïL) with gentle shaking. Following dissociation, the MNP-mAb conjugates were magnetically isol.....
Document: Sample preparation was carried out as described above. MNP-mAb conjugates were added to 500 ïL of the processed samples for 5 min at room temperature while shaking. After the reaction solution was magnetically removed from the supernatant using a magnet, which was discarded, ENR was dissociated from the MNP-mAb conjugatesby adding 100% methanol (500 ïL) with gentle shaking. Following dissociation, the MNP-mAb conjugates were magnetically isolated, and levels of ENR in the supernatant were measured using HPLC.
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