Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_36
Snippet: As a light molecule with a molecular mass of 359.4, enrofloxacin must be conjugated with a carrier protein to elicit the immune response, which an animal uses to produce the desirable antibodies. Szurodoki et al. indicated that,among the carrier proteins for immunogens, KLH is generally a potent immunogen [28] . KLH conjugate was also found to much more accurately stimulate the corresponding region of the protein and to induce the synthesis of la.....
Document: As a light molecule with a molecular mass of 359.4, enrofloxacin must be conjugated with a carrier protein to elicit the immune response, which an animal uses to produce the desirable antibodies. Szurodoki et al. indicated that,among the carrier proteins for immunogens, KLH is generally a potent immunogen [28] . KLH conjugate was also found to much more accurately stimulate the corresponding region of the protein and to induce the synthesis of larger amounts of specific antibody than a BSA conjugate [7, 12] . Therefore, we prepared to conjugate ENR with KLH for an immunogen and ENR with BSA for a coating antigen using the NHS method [15, 22, 29] .
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