Title: Membrane insertion of gap junction connexins: polytopic channel forming membrane proteins Document date: 1994_10_2
ID: 1gqffey0_22
Snippet: SiX) cells were grown and infected with recombinant baculovirus as described by Stauffer et al. (1991) with the following modifications. Cells were split into 10-cm tissue culture dishes and grown overnight to reach semiconfluence. Culture medium was replaced by 5 ml of methionine-free EX-CELL 401 DEFICIENT MEDIUM (JRH Biosciences, Lenexa, KS), and 100 #Ci Tran3~S-label (ICN Radiochemicals, Irvine, CA) and recombinant virus was added from a virus.....
Document: SiX) cells were grown and infected with recombinant baculovirus as described by Stauffer et al. (1991) with the following modifications. Cells were split into 10-cm tissue culture dishes and grown overnight to reach semiconfluence. Culture medium was replaced by 5 ml of methionine-free EX-CELL 401 DEFICIENT MEDIUM (JRH Biosciences, Lenexa, KS), and 100 #Ci Tran3~S-label (ICN Radiochemicals, Irvine, CA) and recombinant virus was added from a virus stock. Cells were grown for 40-60 h at 28"C before cells were lysed in 1 ml RIPA buffer. Connexins were immunoprecipitated as described above.
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