Author: Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao
Title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Document date: 2013_12_6
ID: 4vo7n6nh_31
Snippet: Small RNA library preparation and Illumina sequencing. Total RNA of HSCs and bone marrow-derived DC subsets was isolated using mirVana miRNA Isolation Kit (Ambion) according to the manufacturer's instructions. After PAGE purification of small RNA molecules under 30 bases and ligation of a pair of adaptors to their 5 0 and 3 0 ends, the small RNA molecules were amplified and sequenced on an Illumina platform according to the manufacturer's instruc.....
Document: Small RNA library preparation and Illumina sequencing. Total RNA of HSCs and bone marrow-derived DC subsets was isolated using mirVana miRNA Isolation Kit (Ambion) according to the manufacturer's instructions. After PAGE purification of small RNA molecules under 30 bases and ligation of a pair of adaptors to their 5 0 and 3 0 ends, the small RNA molecules were amplified and sequenced on an Illumina platform according to the manufacturer's instructions 36 .
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