Author: Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao
Title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Document date: 2013_12_6
ID: 4vo7n6nh_35
Snippet: ChIP assay. The cells were crosslinked and processed according to the UpState Chromatin Immunoprecipitation (ChIP) Assay Kit protocol (UpState 17-295). Antibodies to acetyl-Histone H3 (Millipore 06-599), Smad3 (Cell Signaling 9523) and control IgG (Santa Cruz sc-2027) were used at 5 mg per immunoprecipitation. Ten microlitres of sonicated but pre-immunoprecipitated DNA from each sample was used as input control. ChIP results were analysed by real.....
Document: ChIP assay. The cells were crosslinked and processed according to the UpState Chromatin Immunoprecipitation (ChIP) Assay Kit protocol (UpState 17-295). Antibodies to acetyl-Histone H3 (Millipore 06-599), Smad3 (Cell Signaling 9523) and control IgG (Santa Cruz sc-2027) were used at 5 mg per immunoprecipitation. Ten microlitres of sonicated but pre-immunoprecipitated DNA from each sample was used as input control. ChIP results were analysed by real-time PCR. All results were normalized to input in each sample. All primers were listed in Supplementary Data 3.
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