Author: ALFRED, Niyokwishimira; LIU, Huan; LI, Mu Lan; HONG, Shao Feng; TANG, Hai Bo; WEI, Zu Zhang; CHEN, Ying; LI, Fa Kai; ZHONG, Yi Zhi; HUANG, Wei Jian
Title: Molecular epidemiology and phylogenetic analysis of diverse bovine astroviruses associated with diarrhea in cattle and water buffalo calves in China Document date: 2015_2_13
ID: 029hqc82_6
Snippet: Nucleic acid extraction and cDNA bank synthesis: RNA was extracted from 300 µl of the supernatant obtained from each rectal swab using the RNAiso PLUS kit (Takara Bio, Inc., Dalian, China) following the manufacturer's instructions. Extracted RNA was eluted in 35 µl of RNase-free H 2 O (Takara Bio, Inc.) and used for first strand cDNA bank synthesis with the PrimeScript II 1st strand cDNA Synthesis kit (Takara Bio, Inc.) in accordance with the m.....
Document: Nucleic acid extraction and cDNA bank synthesis: RNA was extracted from 300 µl of the supernatant obtained from each rectal swab using the RNAiso PLUS kit (Takara Bio, Inc., Dalian, China) following the manufacturer's instructions. Extracted RNA was eluted in 35 µl of RNase-free H 2 O (Takara Bio, Inc.) and used for first strand cDNA bank synthesis with the PrimeScript II 1st strand cDNA Synthesis kit (Takara Bio, Inc.) in accordance with the manufacturer's instructions.
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