Author: Pipirou, Zoi; Powlesland, Alex S; Steffen, Imke; Pöhlmann, Stefan; Taylor, Maureen E; Drickamer, Kurt
Title: Mouse LSECtin as a model for a human Ebola virus receptor Document date: 2011_1_21
ID: 41i20yuy_24
Snippet: The polymerase chain reaction was carried out using a mouse cDNA panel and Advantage 2 DNA polymerase from ClonTech-Takara Bio Europe (Saint-Germain-en-Laye, France) under the standard reaction conditions described by the manufacturer. Forward and reverse oligonucleotide primers, obtained from Invitrogen (Paisley, UK), were 5ccagggctggacgccaccaccacc-3′ and 5′-ctggggtcactaaagcatgcact ggtcagg-3′. Following an initial denaturation at 95°C for.....
Document: The polymerase chain reaction was carried out using a mouse cDNA panel and Advantage 2 DNA polymerase from ClonTech-Takara Bio Europe (Saint-Germain-en-Laye, France) under the standard reaction conditions described by the manufacturer. Forward and reverse oligonucleotide primers, obtained from Invitrogen (Paisley, UK), were 5ccagggctggacgccaccaccacc-3′ and 5′-ctggggtcactaaagcatgcact ggtcagg-3′. Following an initial denaturation at 95°C for 1 min, 40 cycles of 95°C for 30 s and 68°C for 1 min were executed, and the products were resolved on a 2% agarose gel containing ethidium bromide.
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