Selected article for: "background signal and sample value"

Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays
  • Document date: 2017_9_20
  • ID: 7t1o19kn_33
    Snippet: These data indicate that, although each plasmid in M-NAPPA is present at one-fifth the amount present in standard NAPPA, there was no significant difference of protein display levels between the arrays (p-value = 0.36, paired sample t-test). In addition, background signals that resulted from non-specific antibody binding were comparable between the platforms, demonstrating that multiplexing does not result in an accumulation of background signal .....
    Document: These data indicate that, although each plasmid in M-NAPPA is present at one-fifth the amount present in standard NAPPA, there was no significant difference of protein display levels between the arrays (p-value = 0.36, paired sample t-test). In addition, background signals that resulted from non-specific antibody binding were comparable between the platforms, demonstrating that multiplexing does not result in an accumulation of background signal that could contribute to the identification of false positives ( Figure S5) . Therefore, we randomly mixed different gene plasmids in the following M-NAPPA studies.

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