Selected article for: "protein expression and recombinant protein"
Author: Kim, Sae-Hae; Cho, Byeol-Hee; Lee, Kyung-Yeol; Jang, Yong-Suk
Title: N-terminal Domain of the Spike Protein of Porcine Epidemic Diarrhea Virus as a New Candidate Molecule for a Mucosal Vaccine
  • Document date: 2018_6_15
    • ID: 4vx7ez7s_25
    Snippet: The GST gene, which is a part of the pGEX expression vector, originates from eukaryotic organisms, and GST aids in the stable expression of fusion proteins, because it rapidly folds into a stable conformation during gene translation (23) . The NTD 231-501 gene of the PEDV S1 protein was inserted into the pGEX 4T-1 expression vector containing a thrombin recognition site for cleaving the target protein. After expressing the construct in E. coli (B.....
    Document: The GST gene, which is a part of the pGEX expression vector, originates from eukaryotic organisms, and GST aids in the stable expression of fusion proteins, because it rapidly folds into a stable conformation during gene translation (23) . The NTD 231-501 gene of the PEDV S1 protein was inserted into the pGEX 4T-1 expression vector containing a thrombin recognition site for cleaving the target protein. After expressing the construct in E. coli (BL21 Star (DE3)), the recombinant NTD 231-501 protein yield was 20 mg/L. Although host infection by PEDV begins with the interaction between the PEDV S1 CTD and APN of host cells, recognition of the sugar moiety by the PEDV S1 NTD is also critical in PEDV infection in host cells (8) . Additionally, it was recently reported that knockout of porcine APN in swine testicle (ST) cells did not inhibit PEDV infection (24) . Consequently, we were interested in determining the function of the NTD of the PEDV S protein. We found that the recombinant NTD 231-501 protein produced in this study specifically binds to mucin and to the apical area of M cells.

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