Author: Lee, Na-Ra; Kwon, Hyun-Mi; Park, Kkothanahreum; Oh, Sangtaek; Jeong, Yong-Joo; Kim, Dong-Eun
Title: Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13 Document date: 2010_7_29
ID: 1k99yv4i_3
Snippet: Helicases are molecular motor proteins that translocate along the nucleic acid and separate double-stranded (ds) nucleic acid into two ss nucleic acids using the energy generated from nucleoside triphosphate (NTP) hydrolysis (22) (23) (24) . In choosing duplex nucleic acids as substrates for unwinding, most known helicases unwind only one type of nucleic acid, either the DNA or RNA duplex. However, SCV helicase nsP13 has been shown to display act.....
Document: Helicases are molecular motor proteins that translocate along the nucleic acid and separate double-stranded (ds) nucleic acid into two ss nucleic acids using the energy generated from nucleoside triphosphate (NTP) hydrolysis (22) (23) (24) . In choosing duplex nucleic acids as substrates for unwinding, most known helicases unwind only one type of nucleic acid, either the DNA or RNA duplex. However, SCV helicase nsP13 has been shown to display activity on different types of nucleic acid substrates. The helicase nsP13 can unwind both RNA and DNA duplexes with a 5 0 -to 3 0 -polarity, in which the presence of 5 0 ss overhang is absolutely required to unwind DNA duplexes (6, 7) . Furthermore, it has been observed that both ss RNA and DNA stimulated the ATPase activity of the helicase to the same degree (7, 25) . Thus, it is highly important to understand the duplex nucleic acid unwinding mechanism of the nsP13 helicase with respect to putative controlling factor(s) in nucleic acid unwinding.
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