Author: Kim, Sang Chan; Park, Sook Jahr; Lee, Jong Rok; Seo, Jung Cheol; Yang, Chae Ha; Byun, Sung Hui
Title: Cytoprotective Activity of Glycyrrhizae radix Extract Against Arsenite-induced Cytotoxicity Document date: 2007_3_25
ID: 0c5p8sjk_11
Snippet: H4IIE cells were plated at a density of 5 Â 10 4 cells per well in 96-well plates to determine cytotoxicity induced by heavy metals. Cells were exposed to As at concentrations of 50-800 mM at 37 C under 5% CO 2 . Cells were serum-starved for 12 h treated with licorice for 12 h and exposed to As and licorice for another 12 h. After cell incubation, viable cells were stained with MTT (0.5 mg ml À1 , 4 h). The media were then removed and formazan .....
Document: H4IIE cells were plated at a density of 5 Â 10 4 cells per well in 96-well plates to determine cytotoxicity induced by heavy metals. Cells were exposed to As at concentrations of 50-800 mM at 37 C under 5% CO 2 . Cells were serum-starved for 12 h treated with licorice for 12 h and exposed to As and licorice for another 12 h. After cell incubation, viable cells were stained with MTT (0.5 mg ml À1 , 4 h). The media were then removed and formazan crystals produced in viable cells were dissolved by adding 200 ml of dimethylsulfoxide (DMSO). Absorbance was measured at 570 nm using a Titertek Multiskan Automatic ELISA microplate reader (Model MCC/340, Huntsville, AL, USA). Cell viability was defined relative to untreated control cells [i.e. viability (% control) ¼ 100 Â (absorbance of treated sample)/ (absorbance of control)].
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