Author: Kim, Sang Chan; Park, Sook Jahr; Lee, Jong Rok; Seo, Jung Cheol; Yang, Chae Ha; Byun, Sung Hui
Title: Cytoprotective Activity of Glycyrrhizae radix Extract Against Arsenite-induced Cytotoxicity Document date: 2007_3_25
ID: 0c5p8sjk_15
Snippet: H4IIE cells were pre-treated with licorice for 12 h, and then incubated with licorice with or without 400 uM of As for the next 12 h. After washing the cells with PBS, they were lysed in a solution containing 50 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA and 1% NP-40. The supernatants were incubated with 1% SDS and 2.5 mg ml À1 RNase A for 2 h at 37 C, and then incubated with 0.5 mg ml À1 proteinase K at 42 C. DNA was extracted with phenol-chloroform-i.....
Document: H4IIE cells were pre-treated with licorice for 12 h, and then incubated with licorice with or without 400 uM of As for the next 12 h. After washing the cells with PBS, they were lysed in a solution containing 50 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA and 1% NP-40. The supernatants were incubated with 1% SDS and 2.5 mg ml À1 RNase A for 2 h at 37 C, and then incubated with 0.5 mg ml À1 proteinase K at 42 C. DNA was extracted with phenol-chloroform-isoamylalcohol (25:24:1), and precipitated with 0.3 M sodium acetate and 2.5 volumes of ethanol. DNA samples (20 mg) were electrophoretically separated in 1.5% agarose gel containing 0.1 mg ml À1 ethidium bromide.
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