Selected article for: "cell level and gene expression"
Author: Sousa, Carole; Golebiewska, Anna; Poovathingal, Suresh K; Kaoma, Tony; Pires-Afonso, Yolanda; Martina, Silvia; Coowar, Djalil; Azuaje, Francisco; Skupin, Alexander; Balling, Rudi; Biber, Knut; Niclou, Simone P; Michelucci, Alessandro
Title: Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures
  • Document date: 2018_9_11
    • ID: 0662rivp_23
    Snippet: Gene expression levels of Trem2 and Tyrobp were already shown in Fig. 2A at the bulk level and confirmed at single-cell resolution. As suggested, we added Mef2c to Fig. 2A . Thus, we displayed the decrease of the expression levels of Mef2c, Trem2 and Tyrobp by microglia isolated from LPSinjected mice compared to saline-injected mice. As recommended in minor point 3d "In vitro experiments could also be used to validate findings from the sequencing.....
    Document: Gene expression levels of Trem2 and Tyrobp were already shown in Fig. 2A at the bulk level and confirmed at single-cell resolution. As suggested, we added Mef2c to Fig. 2A . Thus, we displayed the decrease of the expression levels of Mef2c, Trem2 and Tyrobp by microglia isolated from LPSinjected mice compared to saline-injected mice. As recommended in minor point 3d "In vitro experiments could also be used to validate findings from the sequencing experiments", we took advantage of primary cells to analyse Socs3 at gene expression level by qPCR and investigate STAT3 phosphorylation levels by Western blot. Microglial cells treated with LPS showed increased amounts of STAT3 phosphorylation along with up-regulation of Socs3 expression levels compared to untreated cells (Appendix Fig. S1 ).

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