Selected article for: "cell surface and HA cell surface expression"
Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity
  • Document date: 1998_6_15
    • ID: 78fjem8s_62
    Snippet: We invoke the phenotype of the double Pro-substituted mutant, V55P/S71P, as our strongest evidence for the importance of the spring-loaded conformational change: no fusion is observed with V55P/S71P for at least 1 h at pH 5 and 37ЊC. Nonetheless, V55P/S71P behaves like wt-HA in all of its biochemical properties analyzed: trimer formation, cell surface expression, cleavage of HA0, RBC binding, reactivity with an antibody against the major antigen.....
    Document: We invoke the phenotype of the double Pro-substituted mutant, V55P/S71P, as our strongest evidence for the importance of the spring-loaded conformational change: no fusion is observed with V55P/S71P for at least 1 h at pH 5 and 37ЊC. Nonetheless, V55P/S71P behaves like wt-HA in all of its biochemical properties analyzed: trimer formation, cell surface expression, cleavage of HA0, RBC binding, reactivity with an antibody against the major antigenic site, and resistance to proteinase K at neutral pH. The only noted biochemical difference in V55P/S71P is an upward shift ‫6.0ف(‬ U) in its pH dependence of the conformational change, a phenotype seen previously for other fusion-competent HA mutants (Steinhauer et al., 1996) . The lack of fusion activity of V55P/S71P correlates well with its predicted (COILS 2 score ϭ 1.31) and observed (see below) impairment in helix formation.

    Search related documents:
    Co phrase search for related documents
    • antigenic site and cell surface expression: 1, 2, 3
    • antigenic site and conformational change: 1, 2, 3
    • antigenic site and conformational change pH dependence: 1, 2
    • antigenic site and fusion activity: 1, 2, 3
    • antigenic site and major antigenic site: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • antigenic site and major antigenic site antibody: 1, 2, 3, 4
    • antigenic site and pH dependence: 1, 2
    • cell surface and conformational change: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cell surface and conformational change pH dependence: 1, 2
    • cell surface and fusion activity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cell surface and HA mutant: 1, 2, 3
    • cell surface and helix formation: 1
    • cell surface and major antigenic site: 1, 2, 3, 4, 5, 6, 7
    • cell surface and major antigenic site antibody: 1, 2, 3, 4
    • cell surface and pH dependence: 1, 2, 3, 4, 5, 6
    • cell surface expression and conformational change: 1, 2, 3, 4, 5
    • cell surface expression and conformational change pH dependence: 1, 2
    • cell surface expression and fusion activity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • cell surface expression and HA mutant: 1