Author: Munday, Diane C.; Emmott, Edward; Surtees, Rebecca; Lardeau, Charles-Hugues; Wu, Weining; Duprex, W. Paul; Dove, Brian K.; Barr, John N.; Hiscox, Julian A.
Title: Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus Document date: 2010_7_20
ID: 2zhaknbi_30
Snippet: teomes were resolved to study the interaction of HRSV with the host cell. IPA was used to assign identified proteins into different molecular and cellular functional classes (see supplemental Table 8 for definitions) based upon the underlying biological evidence from the curated Ingenuity Pathways Analysis literature database. The relative proportions of proteins represented in each separate functional class in the nuclear and cytoplasmic proteom.....
Document: teomes were resolved to study the interaction of HRSV with the host cell. IPA was used to assign identified proteins into different molecular and cellular functional classes (see supplemental Table 8 for definitions) based upon the underlying biological evidence from the curated Ingenuity Pathways Analysis literature database. The relative proportions of proteins represented in each separate functional class in the nuclear and cytoplasmic proteomes of A549 cells are shown in Fig. 2 . The data indicated that the proteomes differed between the nuclear and cytoplasmic fractions. As would be expected, a greater proportion of proteins involved in gene expression, post-transcriptional modification of RNA, and RNA trafficking were present in the nuclear fraction than in the cytoplasmic fraction, and a greater proportion of proteins involved in protein degradation and lipid metabolism were present in the cytoplasmic fraction than in the nuclear fraction.
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