Author: Bancroft, Tara; DeBuysscher, Blair L.; Weidle, Connor; Schwartz, Allison; Wall, Abigail; Gray, Matthew D.; Feng, Junli; Steach, Holly R.; Fitzpatrick, Kristin S.; Gewe, Mesfin M.; Skog, Patrick D.; Doyle-Cooper, Colleen; Ota, Takayuki; Strong, Roland K.; Nemazee, David; Pancera, Marie; Stamatatos, Leonidas; McGuire, Andrew T.; Taylor, Justin J.
Title: Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes Document date: 2019_10_7
ID: 63yvpuqx_47
Snippet: In adoptive transfer experiments, mouse spleen and lymph node cells were labeled with 2.5 µg/ml GL7 FITC (BD Biosciences), 2 µg/ml anti-CD45.1 PerCP-Cy5.5 (A20; BioLegend), 2 µg/ml anti-CD38 Alexa Fluor 700 (90; eBioscience), 2 µg/ml anti-CD45.2 PE-Cy7 (104; BioLegend), 2 µg/ml anti-CD3 BV510 (145-2C11; BD Biosciences), 2 µg/ml anti-F4/80 BV510 (BM8; BioLegend), 2 µg/ml anti-Gr-1 BV510 (RB6-8C5; BD Biosciences), 2 µg/ml anti-B220 BV786 (R.....
Document: In adoptive transfer experiments, mouse spleen and lymph node cells were labeled with 2.5 µg/ml GL7 FITC (BD Biosciences), 2 µg/ml anti-CD45.1 PerCP-Cy5.5 (A20; BioLegend), 2 µg/ml anti-CD38 Alexa Fluor 700 (90; eBioscience), 2 µg/ml anti-CD45.2 PE-Cy7 (104; BioLegend), 2 µg/ml anti-CD3 BV510 (145-2C11; BD Biosciences), 2 µg/ml anti-F4/80 BV510 (BM8; BioLegend), 2 µg/ml anti-Gr-1 BV510 (RB6-8C5; BD Biosciences), 2 µg/ml anti-B220 BV786 (RA3-6B2; BD Biosciences), and 2 µg/ml anti-CD19 BUV395 (1D3; BD Biosciences). 1 µg/ml streptavidin-BV650 (BD Biosciences) was also included to detect donor cells that were enriched using anti-CD45.2 biotin.
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