Selected article for: "gel electrophoresis and ImageQuant software"
Author: Mateo, Roberto; Nagamine, Claude M.; Kirkegaard, Karla
Title: Suppression of Drug Resistance in Dengue Virus
  • Document date: 2015_12_15
    • ID: 6bx2nrui_34
    Snippet: Immunoblotting. Cell lysates were separated by gel electrophoresis on 12.5% or 15% SDS-polyacrylamide gels and transferred to Immobilon polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA) for 90 min at 100 V in a Miniprotean III transfer tank (Bio-Rad, Hercules, CA). Immunoblots were incubated with anti-core antibody at a dilution of 1/10,000, followed by incubation with alkaline phosphataseconjugated goat anti-rabbit immunoglob.....
    Document: Immunoblotting. Cell lysates were separated by gel electrophoresis on 12.5% or 15% SDS-polyacrylamide gels and transferred to Immobilon polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA) for 90 min at 100 V in a Miniprotean III transfer tank (Bio-Rad, Hercules, CA). Immunoblots were incubated with anti-core antibody at a dilution of 1/10,000, followed by incubation with alkaline phosphataseconjugated goat anti-rabbit immunoglobulin (Jackson ImmunoResearch, West Grove, PA) at a dilution of 1/20,000. Immunoblots were imaged on a PhosporImager (Bio-Rad), and band quantitation was conducted with ImageQuant software (Bio-Rad).

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