Title: Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway Document date: 1995_10_1
ID: 7oklz2ch_43
Snippet: Based on the acquisition of endo H resistance and the localization of ERGIC-53 mutants expressed in COS cells we have established that both the cytoplasmic and the lumenal domain of the protein are required for correct tar-geting of ERGIC-53. We have further shown under high and confirmed under low expression conditions that the cytoplasmic domain contains three targeting determinants: a functional di-lysine ER-retrieval signal, a determinant RSQ.....
Document: Based on the acquisition of endo H resistance and the localization of ERGIC-53 mutants expressed in COS cells we have established that both the cytoplasmic and the lumenal domain of the protein are required for correct tar-geting of ERGIC-53. We have further shown under high and confirmed under low expression conditions that the cytoplasmic domain contains three targeting determinants: a functional di-lysine ER-retrieval signal, a determinant RSQQE adjacent to the membrane and two COOH-terminal phenylalanines that modulate both.
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