Author: Mao, Changyi; Yu, Zhihui; Li, Chengliang; Jin, Yongguo; Ma, Meihu
Title: The Functional Properties of Preserved Eggs: From Anti-cancer and Anti-inflammatory Aspects Document date: 2018_7_31
ID: 3eeykk89_29
Snippet: Approximately 2×10 6 cells/well of Caco-2 cells were placed in 6-well plates and exposed to 2.5, 5, 10 mg/mL of the GI digests for 24 h. We set the cell without the digests as a blank control. After incubation, the cells were immediately collected and washed 2 times by PBS, then centrifuged at a speed of 2,000×g for 5 min to remove the supernatant. The cells were collected after the lysate was added to the cells at a rate of 100 μL/ 2×10 6 ce.....
Document: Approximately 2×10 6 cells/well of Caco-2 cells were placed in 6-well plates and exposed to 2.5, 5, 10 mg/mL of the GI digests for 24 h. We set the cell without the digests as a blank control. After incubation, the cells were immediately collected and washed 2 times by PBS, then centrifuged at a speed of 2,000×g for 5 min to remove the supernatant. The cells were collected after the lysate was added to the cells at a rate of 100 μL/ 2×10 6 cells, and the remaining steps were performed based on the instructions from caspase-3 activity assay kit.
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