Selected article for: "fuca1 type and wild fuca1 type gene"
Author: Cobucci-Ponzano, Beatrice; Conte, Fiorella; Benelli, Dario; Londei, Paola; Flagiello, Angela; Monti, Maria; Pucci, Piero; Rossi, Mosè; Moracci, Marco
Title: The gene of an archaeal a-l-fucosidase is expressed by translational frameshifting
  • Document date: 2006_8_18
    • ID: 69gftii4_14
    Snippet: The mutants in the slippery sequence of the wild-type gene fucA1 were prepared by site-directed mutagenesis from the vector pGEX-11867/3060, described previously (24, 27) . The synthetic oligonucleotides used (PRIMM) were the following: FucA1sm-rev, 5 0 -TTTAGGTGATATTGGTGTT-CTGGTCTATCT-3 0 ; FucA1sm-fwd, 5 0 -GAACACCAATAT-CACCTAAAGAATTCGGCCCA-3 0 ; FucA1tm-rev, 5 0 -AGG-TGATATTGGTGTTCTGGTCTATCTGGC-3 0 ; FucA1tm-fwd, 5 0 -CCAGAACACCAATATCACCTCAAGA.....
    Document: The mutants in the slippery sequence of the wild-type gene fucA1 were prepared by site-directed mutagenesis from the vector pGEX-11867/3060, described previously (24, 27) . The synthetic oligonucleotides used (PRIMM) were the following: FucA1sm-rev, 5 0 -TTTAGGTGATATTGGTGTT-CTGGTCTATCT-3 0 ; FucA1sm-fwd, 5 0 -GAACACCAATAT-CACCTAAAGAATTCGGCCCA-3 0 ; FucA1tm-rev, 5 0 -AGG-TGATATTGGTGTTCTGGTCTATCTGGC-3 0 ; FucA1tm-fwd, 5 0 -CCAGAACACCAATATCACCTCAAGAACTCGGCCCA-GT-3 0 , where the mismatched nucleotides in the mutagenic primers are underlined. Direct sequencing identified the plasmids containing the desired mutations and the mutant genes, named fucA1 sm and fucA1 tm , were completely re-sequenced.

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