Selected article for: "ethidium bromide and initial denaturation"
Author: Cobucci-Ponzano, Beatrice; Conte, Fiorella; Benelli, Dario; Londei, Paola; Flagiello, Angela; Monti, Maria; Pucci, Piero; Rossi, Mosè; Moracci, Marco
Title: The gene of an archaeal a-l-fucosidase is expressed by translational frameshifting
  • Document date: 2006_8_18
    • ID: 69gftii4_28
    Snippet: For each amplification of the fucA1 gene was used $2500fold more cDNA than that used for the amplification of the 16S rRNA. Controls with no template cDNA were always included. PCR conditions were 15 min at 95 C for initial denaturation, followed by 40 cycles of 10 s at 95 C, 25 s at 56 C and 35 s at 72 C, and a final step of 10 min at 72 C. Product purity was controlled by melting point analysis of setpoints with 0.5 C temperature increase from .....
    Document: For each amplification of the fucA1 gene was used $2500fold more cDNA than that used for the amplification of the 16S rRNA. Controls with no template cDNA were always included. PCR conditions were 15 min at 95 C for initial denaturation, followed by 40 cycles of 10 s at 95 C, 25 s at 56 C and 35 s at 72 C, and a final step of 10 min at 72 C. Product purity was controlled by melting point analysis of setpoints with 0.5 C temperature increase from 72 to 95 C. PCR products were analysed on 2% agarose gels and visualized by ethidium bromide staining.

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