Author: Ma, Yuanmei; Zhang, Yu; Liang, Xueya; Lou, Fangfei; Oglesbee, Michael; Krakowka, Steven; Li, Jianrong
Title: Origin, Evolution, and Virulence of Porcine Deltacoronaviruses in the United States Document date: 2015_3_10
ID: 6o7j5k9n_35
Snippet: Piglets. The animal protocol used in this study was approved by the Institutional Laboratory Animal Care and Use Committee of The Ohio State University (IACUC-OSU). Gnotobiotic (Gn) piglets were derived in a sterile environment via caesarean section from a specific-pathogen-free gravid sow as previously described (33) and housed in germfree isolation units. Piglets were fed a milk-replacement diet (Permalat) and maintained as described elsewhere .....
Document: Piglets. The animal protocol used in this study was approved by the Institutional Laboratory Animal Care and Use Committee of The Ohio State University (IACUC-OSU). Gnotobiotic (Gn) piglets were derived in a sterile environment via caesarean section from a specific-pathogen-free gravid sow as previously described (33) and housed in germfree isolation units. Piglets were fed a milk-replacement diet (Permalat) and maintained as described elsewhere (33) . Ten-day-old healthy conventional piglets were purchased from Hartley Farm, Circleville, OH. These piglets were seronegative for major porcine diseases, including PdCV, PEDV, transmissible gastroenteritis virus (TEGV), porcine circovirus (PCV), and porcine reproductive and respiratory syndrome virus (PRRSV).
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