Heat shock protein 90ß in the Vero cell membrane binds Japanese encephalitis virus

Wang, Yuan; Li, Yan; Ding, Tianbing

Specif ic mAb inhibits HSP9 0β-JEV binding in a dose-dependent manner. When the binding of HSP90β and JEV was ascertained, a virus infection inhibition experiment with rabbit anti-human HSP90β mAb at various concentrations was conducted using qualitative IFA and quantitative FCM. In the IFA experiment, green fluorescence gradually decreased with an increase in the anti-HSP90β mAb concentrations 0, 10 and 20 µg/ml (Fig. 3A) . The serial numbers listed in this table are not one-to-one matched with gel bands indicated by arrows in Fig. 1 . Moreover, the percentages of HSP90β-JEV binding measured by FCM also significantly decreased (P<0.01) from 76.17±0.69% (0 µg/ml anti-HSP90β m Ab) to 19.15±0.35% (20 µg/ml anti-HSP90β mAb) and further to 10.43±0.53% (100 µg/ml anti-HSP90β mAb) compared with the negative controls of 1.70±0.11% ( Fig. 3B and C) . These results clearly demonstrated that a specific anti-HSP90β mAb inhibited HSP90β-JEV binding in a dose-dependent manner.