Title: 2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018 Document date: 2018_10_25
ID: 60ceejq1_154
Snippet: Mitochondria were isolated from liver tissue of a systemically healthy horse by cell lysis and centrifugation. Mitochondria were then fragmented by freeze-thawing and serially diluted from 1:1 to 1:512 in PBS, and added to aliquots of whole blood from 6 healthy horses, mixed 1:1 with RPMI medium, with one aliquot serving as a negative control. Aliquots were incubated rotating at 37 C for 21 hours. The inflammatory response was determined by measu.....
Document: Mitochondria were isolated from liver tissue of a systemically healthy horse by cell lysis and centrifugation. Mitochondria were then fragmented by freeze-thawing and serially diluted from 1:1 to 1:512 in PBS, and added to aliquots of whole blood from 6 healthy horses, mixed 1:1 with RPMI medium, with one aliquot serving as a negative control. Aliquots were incubated rotating at 37 C for 21 hours. The inflammatory response was determined by measuring TNF-α in the supernatant using a murine L929 fibroblast bioassay.
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