2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

In conclusion, the data suggest that 3% HHES support sufficient volume expansion without overloading, return of systemic oxygenation indices and lactate to control level, and the superior resuscitation benefits on acid-base status and inflammatory stability after reperfusion injury than conventional resuscitation fluids. Thus, 3% HHES could be the best alternative solution that compensate dosage disadvantages of conventional crystalloid or colloid administration. Oxidative stress is primarily assessed through the activity of antioxidant enzymes, the concentration of endogenous antioxidants, and byproducts of oxidative damage. F2-isoprostanes, a byproduct of lipid peroxidation, were determined to be the best marker of oxidative injury in a rodent model of oxidative stress, and are stable and easily measured in urine. In people, the gold standard for measurement of urinary F2-isoprostanes is gas chromatography-mass spectrometry (GC-MS), but in animals the majority of studies have utilized enzymelinked immunosorbent assay (ELISA) for measurement of F2-isoprostanes. A previous study compared these two methods in small populations of dogs, cats, horses, and cows. Poor agreement between these methods was identified in dogs, horses, and cows.