Author: Tucci, Paula; Estevez, Verónica; Becco, Lorena; Cabrera-Cabrera, Florencia; Grotiuz, Germán; Reolon, Eduardo; Marín, Mónica
Title: Identification of Leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen Document date: 2016_9_19
ID: 71omypkg_14
Snippet: Specific primers were designed to amplify a portion of LKT which contains the Cterminal domain of the protein. An in silico prediction of the location of linear Bcell epitopes was performed using Bepipred algorithm (Larsen et al., 2006) . As previously reported (Lee et al., 2001) , the portion of LktA with more immunogenic epitopes was the C-terminal domain. Therefore, a sequence including the main predicted epÃtopes (amino acid 713 to 873) was .....
Document: Specific primers were designed to amplify a portion of LKT which contains the Cterminal domain of the protein. An in silico prediction of the location of linear Bcell epitopes was performed using Bepipred algorithm (Larsen et al., 2006) . As previously reported (Lee et al., 2001) , the portion of LktA with more immunogenic epitopes was the C-terminal domain. Therefore, a sequence including the main predicted epÃtopes (amino acid 713 to 873) was selected. M. haemolytica (production strain code B059) was cultured as the seed culture described above.
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