Selected article for: "dna sample and EGFR mutation"
Author: Horiuchi, Sho; Saito, Yuichi; Matsui, Atsuka; Takahashi, Nobumasa; Ikeya, Tomohiko; Hoshi, Eishin; Shimizu, Yoshihiko; Yasuda, Masanori
Title: A novel loop-mediated isothermal amplification method for efficient and robust detection of EGFR mutations
  • Document date: 2020_1_14
    • ID: 4sltubqk_9
    Snippet: LAMP EGFR mutation analysis. The LAMP assay detected 27 EGFR wild types and 32 EGFR mutations (Table II) . Among 32 EGFR mutations, 18 exon 21 L858R point mutations (54.5%), 11 exon 19 deletions (33.3%), 2 simultaneous exon 18 G719X point mutation/exon 20 S768I point mutations (6.1%) and 1 exon 20 SS761I point mutation alone (3.0%) were identified (Table II) . concentration for EGFR mutation detection was 4.8% of DNA sample in Therascreen assay (.....
    Document: LAMP EGFR mutation analysis. The LAMP assay detected 27 EGFR wild types and 32 EGFR mutations (Table II) . Among 32 EGFR mutations, 18 exon 21 L858R point mutations (54.5%), 11 exon 19 deletions (33.3%), 2 simultaneous exon 18 G719X point mutation/exon 20 S768I point mutations (6.1%) and 1 exon 20 SS761I point mutation alone (3.0%) were identified (Table II) . concentration for EGFR mutation detection was 4.8% of DNA sample in Therascreen assay (Table SI) . On the other hand, 0.1% was the minimum concentration in LAMP assay, since LAMP assay demonstrated one success of the detection per 3 tests at the level of 0.1, 0.5 and 1.0% concentrations and all positive per 3 tests at >4.8% concentration (Table SI) .

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