Selected article for: "Ã1 PRF site and frameshift efficiency"

Author: Theis, Corinna; Reeder, Jens; Giegerich, Robert
Title: KnotInFrame: prediction of -1 ribosomal frameshift events
  • Document date: 2008_9_27
  • ID: 4ov0j2u3_3
    Snippet: When two cis-acting signals are present, À1 PRF is most effective: a heptameric slippery site and a stable secondary structure. The slippery site is the location of the actual frameshift event having the consensus sequence X XXY YYZ (triplets are shown for the preshifted reading frame). The structural element follows within a few bases and can be a simple stem-loop structure or a pseudoknot. It is believed that the pseudoknot promotes a higher f.....
    Document: When two cis-acting signals are present, À1 PRF is most effective: a heptameric slippery site and a stable secondary structure. The slippery site is the location of the actual frameshift event having the consensus sequence X XXY YYZ (triplets are shown for the preshifted reading frame). The structural element follows within a few bases and can be a simple stem-loop structure or a pseudoknot. It is believed that the pseudoknot promotes a higher frameshift efficiency, since it is more effective in pausing the ribosome. It is also known that ribosome pausing is a necessary, but not sufficient prerequisite for frameshifting (6) . The exact mechanism, by which the ribosome is brought into a different reading frame is still unknown. Two different models were recently proposed in (7) and (8) .

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