Selected article for: "base call and likelihood model"

Author: Lee, Charlie Wah Heng; Koh, Chee Wee; Chan, Yang Sun; Aw, Pauline Poh Kim; Loh, Kuan Hon; Han, Bing Ling; Thien, Pei Ling; Nai, Geraldine Yi Wen; Hibberd, Martin L.; Wong, Christopher W.; Sung, Wing-Kin
Title: Large-scale evolutionary surveillance of the 2009 H1N1 influenza A virus using resequencing arrays
  • Document date: 2010_2_25
  • ID: 1rhy8td0_36
    Snippet: The presence of nucleotide substitution bias in Nimblegen resequencing arrays has been previously described (15) . However, this knowledge has so far been used only to improve probe design. In this article, we propose a novel method that makes use of nucleotide substitution bias in the array to improve base-calling accuracy and call rate. The key idea is to build a likelihood model of the substitution bias among the probes of non-ambiguous calls .....
    Document: The presence of nucleotide substitution bias in Nimblegen resequencing arrays has been previously described (15) . However, this knowledge has so far been used only to improve probe design. In this article, we propose a novel method that makes use of nucleotide substitution bias in the array to improve base-calling accuracy and call rate. The key idea is to build a likelihood model of the substitution bias among the probes of non-ambiguous calls on the array; then use this to call bases with ambiguous signals.

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