Title: The rubella virus E1 glycoprotein is arrested in a novel post-ER, pre- Golgi compartment Document date: 1992_8_2
ID: 04455ffs_19
Snippet: Chemical cross-linking was done as previously described (18) with slight modifications. CHOE1 cells were radiolabeled as above and incubated with 500 #g/ml DSP (from a fresh DMSO stock) on ice for 30 rain before lysis in PBS (pH 8.0) continuing 1% NP-40. Where indicated, labeled proteins were also cross-linked during cell lysis by including cross-linking agent in the lysis buffer. After 30 min lysates were adjusted to 60 mM glycine to inactivate .....
Document: Chemical cross-linking was done as previously described (18) with slight modifications. CHOE1 cells were radiolabeled as above and incubated with 500 #g/ml DSP (from a fresh DMSO stock) on ice for 30 rain before lysis in PBS (pH 8.0) continuing 1% NP-40. Where indicated, labeled proteins were also cross-linked during cell lysis by including cross-linking agent in the lysis buffer. After 30 min lysates were adjusted to 60 mM glycine to inactivate the cross-linker. Protease inhibitors were also added after the cross-linking reaction. The postnuclear lysates were immunoprecipitated as above.
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