Title: The rubella virus E1 glycoprotein is arrested in a novel post-ER, pre- Golgi compartment Document date: 1992_8_2
ID: 04455ffs_23
Snippet: For routine morphology studies, cells were fixed (1 h) with 2 % glutaraldehyde, 3% paraformaldehyde in 100 mM cacodylate-HCl buffer, pH 7.2, scraped from the culture dish, and pelleted in a microfuge. The cell pellets were then postfixed (1 h) in 2% OsO4 in the same buffer, stained in block (2 h) with 2% uranyl acetate (pH 6.0), dehydrated in graded ethanols, and embedded in Epon......
Document: For routine morphology studies, cells were fixed (1 h) with 2 % glutaraldehyde, 3% paraformaldehyde in 100 mM cacodylate-HCl buffer, pH 7.2, scraped from the culture dish, and pelleted in a microfuge. The cell pellets were then postfixed (1 h) in 2% OsO4 in the same buffer, stained in block (2 h) with 2% uranyl acetate (pH 6.0), dehydrated in graded ethanols, and embedded in Epon.
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