Title: Membrane protein retention in the yeast Golgi apparatus: dipeptidyl aminopeptidase A is retained by a cytoplasmic signal containing aromatic residues Document date: 1993_6_2
ID: 0pz80zbg_12
Snippet: A STE/3 disruption plasmid was constructed by inserting a ~4-kbp BcU fragment of the LEU2 gene into BclI sites at positions 1235 and 2843 of the STE/3 gene (the A of the ATG is nucleotide 163; removing 1.6-kbp of coding sequence), which had been cloned into pBR322 as a 7.2-kbp BamHI fragment, giving rise to plasmid pSL349, stel3A strains were constructed by transforming (Ito et al., 1983) leu2 yeast strains to Leu + with the ~9.5kbp BamHI ste13A:.....
Document: A STE/3 disruption plasmid was constructed by inserting a ~4-kbp BcU fragment of the LEU2 gene into BclI sites at positions 1235 and 2843 of the STE/3 gene (the A of the ATG is nucleotide 163; removing 1.6-kbp of coding sequence), which had been cloned into pBR322 as a 7.2-kbp BamHI fragment, giving rise to plasmid pSL349, stel3A strains were constructed by transforming (Ito et al., 1983) leu2 yeast strains to Leu + with the ~9.5kbp BamHI ste13A::LEU2 fragment from pSL349.
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