The v-sis oncoprotein loses transforming activity when targeted to the early Golgi complex

For example, the sis-E1 contsruct required synthesis of sense and antisense oligonucleotides, designated D369 and D370, respectively. The sequence of D369 is: 5'GTG.ACC.TAT.AAC.CTG.TTC.ATC.ACC.GCC.TTC. CTG.CTG.TTC.CTG. AC C. ATC. ATC.CTG.CAG.TAT.GC~.TAT.GCC.ACC. CGG.AGC.AAG.TAA.T y. This oligonucleotide encodes the amino acid sequence VTYNLFITAFLLFLTIILQYGYATRSK*, where the first two amino acids correspond to amino acids 238 and 239 of v-sis, which lie at the BstEII restriction site. The remaining sequence corresponds to amino acids 21--45 of El, which encompasses the first transmembrane domain (Swift and Machamer, 1991) . Mutant versions of sis-E1, designated sis-El(Ql) and sis-El(ins), were also constructed in the same manner. The complementary oligonucleotides encoding sis-El(ins) are designated I)419 and I)420 and code for the sequence VTYNLFITAFLIILFLTIILQYGYATRSK* which contains an insertion of two lie residues between amino acids 29 and 30 of E1 (shown in bold italics). The oligonucleotides encoding the other mutant, sis-El(Ql) are designated D421 and D422 and code for the sequence VTYNLFITAFLLFLTIIL1YGYATRSK* which contains a mutation of Glu37 to lie (shown in bold italics).