Author: Park, Jeong-In; Song, Kyung-Hee; Jung, Seung-Youn; Ahn, Jiyeon; Hwang, Sang-Gu; Kim, Joon; Kim, Eun Ho; Song, Jie-Young
Title: Tumor-Treating Fields Induce RAW264.7 Macrophage Activation Via NK-?B/MAPK Signaling Pathways Document date: 2019_8_11
ID: 65s65ojc_13
Snippet: Reactive oxygen species (ROS) levels in macrophages were monitored using the fluorescent ROS indicator C2 0 ,7 0 -dichlorodihydrofluorescein diacetate (5 mmol/L; Molecular Probes, Eugene, Oregon). Cell-associated fluorescence was detected by fluorescence-activated cell signaling (FACS) using a FACS-Calibur flow cytometer (BD Biosciences, San Diego, California) with FlowJo software V 7.2.5 (Tree Star Inc, Ashland, Orogen). Images of fluorescent ce.....
Document: Reactive oxygen species (ROS) levels in macrophages were monitored using the fluorescent ROS indicator C2 0 ,7 0 -dichlorodihydrofluorescein diacetate (5 mmol/L; Molecular Probes, Eugene, Oregon). Cell-associated fluorescence was detected by fluorescence-activated cell signaling (FACS) using a FACS-Calibur flow cytometer (BD Biosciences, San Diego, California) with FlowJo software V 7.2.5 (Tree Star Inc, Ashland, Orogen). Images of fluorescent cells were acquired under a 710 confocal microscope (Carl-Zeiss, Germany).
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