Membrane protein retention in the yeast Golgi apparatus: dipeptidyl aminopeptidase A is retained by a cytoplasmic signal containing aromatic residues

The primary antibodies used for indirect immunofluorescence were as follows: (a) for ALP and A-ALP, an affinity purified rabbit polyclonal antibody against the luminal domain of ALP, which had been preadsorbed against fixed phoSA yeast cells (Raymond et al., 1992) was used; (b) for Kex2p, an affinity purified rabbit polyclonal antibody against Kex2p kindly provided by Robert Fuller was used; (c) for DPAP A, an affinity purified rabbit polyclonal antibody against the luminal domain of DPAP A, which had been preadsorbed against fixed stel3A yeast cells (Roberts et al., 1992) was used; and (d) for the 60-kD vacuolar proton-translocating ATPase (V-ATPase) subunit, the mouse monoclonal antibody 13Dll (Kane et al., 1992) was used.