Author: Riede, O; Seifert, K; Oswald, D; Endmann, A; Hock, C; Winkler, A; Salguero, F J; Schroff, M; Croft, S L; Juhls, C
Title: Preclinical safety and tolerability of a repeatedly administered human leishmaniasis DNA vaccine Document date: 2015_4_30
ID: 4eyn7pjq_25
Snippet: Quantitative analyses of DNA vector amounts were performed at IMGM Laboratories (Martinsried, Germany). Total DNA was extracted from Safety and tolerability of a leishmaniasis DNA vaccine O Riede et al ~20 mg tissue or up to 280 μl blood, respectively, using the DNeasy Blood&Tissue Kit (Qiagen, Hilden, Germany) and stored at − 20°C. A TaqMan-based quantitative realtime-PCR assay was established and characterized using a standard curve of LEIS.....
Document: Quantitative analyses of DNA vector amounts were performed at IMGM Laboratories (Martinsried, Germany). Total DNA was extracted from Safety and tolerability of a leishmaniasis DNA vaccine O Riede et al ~20 mg tissue or up to 280 μl blood, respectively, using the DNeasy Blood&Tissue Kit (Qiagen, Hilden, Germany) and stored at − 20°C. A TaqMan-based quantitative realtime-PCR assay was established and characterized using a standard curve of LEISHDNAVAX ranging from 10 vector copies per reaction to 1.05 × 10 11 vector copies per reaction with 200 ng genomic DNA from the livers of naive animals as background.
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