Author: Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao
Title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Document date: 2013_12_6
ID: 4vo7n6nh_34
Snippet: RNA quantification. Real-time qRT-PCR assay was performed to measure the expression of miRNA using stem-loop RT primer and expression of pri-miRNA using oligo(dT) RT primer. Expressions of miRNA and pri-miRNA were normalized to the expression of U6 and b-actin in each sample, respectively. All primers were listed in Supplementary Data 3......
Document: RNA quantification. Real-time qRT-PCR assay was performed to measure the expression of miRNA using stem-loop RT primer and expression of pri-miRNA using oligo(dT) RT primer. Expressions of miRNA and pri-miRNA were normalized to the expression of U6 and b-actin in each sample, respectively. All primers were listed in Supplementary Data 3.
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