Selected article for: "gene fragment and rpoB subunit gene RNA polymerase"
Author: Robinson, Lary A; Smith, Prudence; SenGupta, Dhruba J; Prentice, Jennifer L; Sandin, Ramon L
Title: Molecular analysis of sarcoidosis lymph nodes for microorganisms: a case–control study with clinical correlates
  • Document date: 2013_12_21
    • ID: 3unap1o9_120
    Snippet: By searching the Moffitt Cancer Center surgical pathology database between January 1, 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 Inhibition was ruled out by checking the ability of exogenously added target DNA to be amplified in the same PCR mix that contained DNA ex.....
    Document: By searching the Moffitt Cancer Center surgical pathology database between January 1, 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 Inhibition was ruled out by checking the ability of exogenously added target DNA to be amplified in the same PCR mix that contained DNA extracted from clinical specimen. The 16S gene fragment was amplified as previously described. 8 The hsp65 gene was amplified using TB11 and TB12 primers, and the RNA polymerase subunit gene (rpoB) was amplified using MF and MR primers. 9 The amplified products were then sequenced using the

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