Author: Robinson, Lary A; Smith, Prudence; SenGupta, Dhruba J; Prentice, Jennifer L; Sandin, Ramon L
Title: Molecular analysis of sarcoidosis lymph nodes for microorganisms: a case–control study with clinical correlates Document date: 2013_12_21
ID: 3unap1o9_38
Snippet: Nevertheless, the molecular approach has distinct limitations including possible false-positive results secondary to contaminated PCR reagents, the paraffin imbedding process, or postembedding handling and processing of the paraffin block. However in our study, thirty control lymph nodes were processed in an identical manner and bacterial DNA was detected in only 2/30 (6.6%), significantly less than the sarcoidosis nodes (36.7%, p = 0.00516), sug.....
Document: Nevertheless, the molecular approach has distinct limitations including possible false-positive results secondary to contaminated PCR reagents, the paraffin imbedding process, or postembedding handling and processing of the paraffin block. However in our study, thirty control lymph nodes were processed in an identical manner and bacterial DNA was detected in only 2/30 (6.6%), significantly less than the sarcoidosis nodes (36.7%, p = 0.00516), suggesting that contamination is unlikely to account for the findings.
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